mouse testicular tm3 cell line (Korean Cell Line Bank)
86
Structured Review
Korean Cell Line Bank
mouse testicular tm3 cell line
Mouse Testicular Tm3 Cell Line, supplied by Korean Cell Line Bank, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cell+line+tm3/cells+levels+leydig+testosterone+tm3/pmc13049662-91-1-15
Average 86 stars, based on 1 article reviews
Mouse Testicular Tm3 Cell Line, supplied by Korean Cell Line Bank, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cell+line+tm3/cells+levels+leydig+testosterone+tm3/pmc13049662-91-1-15
Average 86 stars, based on 1 article reviews
mouse testicular tm3 cell line - by Bioz Stars,
2026-10
86/100 stars
Images
1) Product Images from "AMPK inhibition and elevated angiogenin are associated with tRNA fragmentation in the male germline exposed to a high-fat diet"
Article Title: AMPK inhibition and elevated angiogenin are associated with tRNA fragmentation in the male germline exposed to a high-fat diet
Journal: Molecular Metabolism
doi: 10.1016/j.molmet.2026.102350
Figure Legend Snippet: AMPK inhibition-induced Angiogenin is accompanied by alteration of tRFs profile in vitro. A Western blot results of proteins related with AMPK-mTOR pathways in TM3, TM4, and NIH/3T3 cell lines using compound C (CC), rapamycin (Rapa), and MHY1485 (MHY) in TM3, TM4, and NIH/3T3 cell lines (left to right). The number represents the densiometric comparison of the expression of pAMPK and pmTOR proteins against AMPK and mTOR, respectively. B Angiogenin gene expression. Gene expression was quantified after 12 h of treatment. mRNA expression level was normalized to Actin . Data are represented as mean ± standard deviation. Statistical analysis was performed using One-way ANOVA with Tukey post-hoc test. Different letters are significantly different. C Northern blot results of tRF3-Pro and Val in mouse testis cell lines. Each sample RNAs were extracted from both TM3 and TM4 cells with CC treatment and overexpression of Angiogenin (Ang OE). Each intact tRNA was shown as a control. Band intensities of Northern blot are represented as bar graph after quantification using Image J software. Data are presented as mean ± standard deviation. One-way ANOVA was used for statistical analysis, and the different alphabets with Tukey post-hoc test. D Distribution of read counts based on small RNA type. E Distribution of reads assigned to GtRNAdb in tRF subtypes. The total bar height is proportional to the total read count for each group. Percentages indicate the relative proportion of each tRF subtype within the corresponding group, and n represents the actual read count number for each subtype. tRF5, reads derived from tRNA 5′ end among reads aligned to GtRNAdb; tRF3, reads derived from tRNA 3′ end among reads aligned to GtRNAdb; other, not included in the previous two groups. F Histogram showing the distribution of log 2 (Fold change) using reads aligned to GtRNAdb. Green, CC-treated versus non-treated; Yellow, ANG overexpression versus control. G Volcano plot depicting significantly changed tRFs in TM4 cell lines. Results from CC-treated versus non-treated are represented as circles, and results from ANG overexpression versus control are represented as triangles. n = 2 for each group.
Techniques Used: Inhibition, In Vitro, Western Blot, Comparison, Expressing, Gene Expression, Standard Deviation, Northern Blot, Over Expression, Control, Software, Derivative Assay
Related Articles
CCK-8 Assay:Article Title: Differential nanoreprotoxicity of silver nanoparticles in male somatic cells and spermatogonial stem cells Article Snippet: Cell Counting:Article Title: Differential nanoreprotoxicity of silver nanoparticles in male somatic cells and spermatogonial stem cells Article Snippet: Concentration Assay:Article Title: Differential nanoreprotoxicity of silver nanoparticles in male somatic cells and spermatogonial stem cells Article Snippet: Standard Deviation:Article Title: Differential nanoreprotoxicity of silver nanoparticles in male somatic cells and spermatogonial stem cells Article Snippet: Activity Assay:Article Title: Differential nanoreprotoxicity of silver nanoparticles in male somatic cells and spermatogonial stem cells Article Snippet: Fluorescence:Article Title: Differential nanoreprotoxicity of silver nanoparticles in male somatic cells and spermatogonial stem cells Article Snippet: Transmission Assay:Article Title: Differential nanoreprotoxicity of silver nanoparticles in male somatic cells and spermatogonial stem cells Article Snippet: Electron Microscopy:Article Title: Differential nanoreprotoxicity of silver nanoparticles in male somatic cells and spermatogonial stem cells Article Snippet: Expressing:Article Title: Differential nanoreprotoxicity of silver nanoparticles in male somatic cells and spermatogonial stem cells Article Snippet: Gene Expression:Article Title: Differential nanoreprotoxicity of silver nanoparticles in male somatic cells and spermatogonial stem cells Article Snippet: Staining:Article Title: Differential nanoreprotoxicity of silver nanoparticles in male somatic cells and spermatogonial stem cells Article Snippet: Flow Cytometry:Article Title: Differential nanoreprotoxicity of silver nanoparticles in male somatic cells and spermatogonial stem cells Article Snippet: Western Blot:Article Title: Differential nanoreprotoxicity of silver nanoparticles in male somatic cells and spermatogonial stem cells Article Snippet: Control:Article Title: Differential nanoreprotoxicity of silver nanoparticles in male somatic cells and spermatogonial stem cells Article Snippet: Reverse Transcription Polymerase Chain Reaction:Article Title: Differential nanoreprotoxicity of silver nanoparticles in male somatic cells and spermatogonial stem cells Article Snippet: Real-time Polymerase Chain Reaction:Article Title: Differential nanoreprotoxicity of silver nanoparticles in male somatic cells and spermatogonial stem cells Article Snippet: Quantitative RT-PCR:Article Title: Differential nanoreprotoxicity of silver nanoparticles in male somatic cells and spermatogonial stem cells Article Snippet: Cell Culture:Article Title: Differential nanoreprotoxicity of silver nanoparticles in male somatic cells and spermatogonial stem cells Article Snippet: Incubation:Article Title: Differential nanoreprotoxicity of silver nanoparticles in male somatic cells and spermatogonial stem cells Article Snippet: FACS:Article Title: Differential nanoreprotoxicity of silver nanoparticles in male somatic cells and spermatogonial stem cells Article Snippet: Modification:Article Title: Differential nanoreprotoxicity of silver nanoparticles in male somatic cells and spermatogonial stem cells Article Snippet: |
